BIOCHEMISTRY II
(a.k.a. Advanced Biochemistry)
Syllabus
Chemistry
48005/A8005 Spring 2008
Room
NAC 6/112
Mon
11:00-12:50
Wed
11:00-11:50
Professors K. Ryan and M. Steinberg
Office
hours:
Ryan: Mon 4:00-5:30 PM; Tues
10:45 AM – 11:45. Office MR-1337; Tel: (212) 650-8132. email:
kr107@sci.ccny.cuny.edu
Steinberg: Mon 2-4 PM, Wed 2-4
PM. Office: MR-629; Tel: (212)
650-8560; Fax: (212) 650-6092; email: marste@sci.ccny.cuny.edu.
Course
Description:
Molecular basis of enzyme action, membranes (transport and transduction),
protein structure, signal transduction, virology, Bioinformatics, genomics,
proteomics, molecular basis of replication, transcription and translation of
genetic information, and immunology
Prerequisites: Chem 45900 3 hr./wk; 3 cr.
Text:
Lehninger
Principles
of Biochemistry: Nelson & Cox, 2005,
4th
Ed. W.H. Freeman & Co., NY. ISBN 0-7167-4339-6
===================================================================================
|
Date |
Topic |
Text |
|
Jan
28 Mon |
Nucleosides
and Nucleotides |
Lehninger
pp.273-284 |
|
Jan
30 Wed |
Nucleic
Acids I |
Lehninger
pp.285-302 |
|
Feb
4 Mon |
Nucleic
Acids II |
Lehninger
pp.306-317 |
|
Feb
6 Wed |
DNA
Replication |
Lehninger pp.950-966 |
|
Feb
11 Mon |
Transcription |
Lehninger
pp.995-1006 |
|
Feb
13 Wed |
RNA
Processing I |
Lehninger
pp.1007-1017, 1020-22, 1025-1030 |
|
Feb
20 Wed |
RNA
Processing II |
“
“ Continued |
|
Feb
25 Mon |
Exam I |
-- |
|
Feb
27 Wed |
micro
RNA |
Handout |
|
Mar
3 Mon |
Antisense,
Ribozymes and RNAi |
Handout |
|
Mar
5 Wed |
Amino
Acid Biosynthesis |
Lehninger
Ch 22, Selected topics |
|
Mar
10 Mon |
Protein
Biosynthesis |
Lehninger
pp.1048-1062 |
|
Mar
12 Wed |
Posttranslational
modifications; Review for mid-term |
Handout |
|
Mar
17 Mon |
Exam II |
-- |
|
Mar
19 Wed |
Immune
and Complement systems |
Lehninger
pp. 174-182, 990-991 |
|
Mar
26 Wed |
The
Blood Clotting System |
Handout |
|
Mar
31 Mon |
G
protein-coupled Receptors |
Lehninger
pp. 433-451, 464-466 |
|
Apr
2 Wed |
Biosignaling
and Insulin |
Lehninger
pp. 421-425, 429-433 |
|
Apr
7 Mon |
Recombinant
DNA Technology |
Lehninger
Chapter 9, pp. 318-338 |
|
Apr
9 Wed |
Recombinant
DNA Technology |
Handout |
|
Apr
14 Mon |
Exam III |
-- |
|
Apr
16 Wed |
Gene
mapping/prokaryotic genome |
Handout |
|
Apr
28 Mon |
Phage
Lambda |
Handout |
|
Apr
30 Wed |
Oncogenes
and retroviruses |
Handout |
|
May
5 Mon |
Oncogenes,
signaling pathways and cell cycle regulation |
Handout |
|
May
7 Wed |
Genetic
Disease |
Handout |
|
May
12 Mon |
Genetic
Disease |
Handout |
|
May
14 Wed |
Exam IV |
-- |
Grading:
based on 4
equally weighted lecture exams. A cumulative final exam will be offered as a
makeup for students who miss a regularly scheduled lecture exam. Extra credit may be available during
the first half of the course - please see Prof. Ryan if interested.
Academic
integrity:
The CCNY policy on academic integrity will be followed. Document is posted on
the CCNY website (CUNY policy on academic integrity—link is at the bottom
of the home page). Make sure you have read the details regarding plagiarism and
cheating, in case you are not clear about the rules of the college. Cases where
academic integrity is compromised will be prosecuted according to these rules.
Course Objectives/Learning Outcomes for A8005/48005
After
completing this course, students should:
1. Know
the structure and basic chemical properties of the nucleosides and nucleotides (a,b,g)
2. Be
able to recognize the various forms of nucleic acids and describe the chemical
basis of heredity (a,b,g)
3. Be
able to contrast and compare gene expression in prokaryotes with that in
eukaryotes (a,b,g)
4. Understand
the processes of transcription, replication and translation, and know the roles
of the major polypeptides involved (a,b,g)
5. Be
able to describe how eukaryotic mRNA processing alters the information of the
genome, and prepares it for gene expression (a,b,g)
6. Compare
the naturally occurring microRNAs with synthetic siRNAs (a,j,b,g)
7. be
able to design a synthetic molecule to knock-down a human gene and thereby
study its function (J)
8. Know
how biochemists make synthetic DNA, RNA and peptides to use in experimental
biochemistry (a,j,b,g)
9. Know
the common post-translational modifications and how they modify protein
function (a,b,g)
10. Be able to apply the basic concepts of
bioinformatics to modern biomolecular modeling (b,d,e)
11. Understand biotechnological approaches
to genetic disease including the genetic basis of cancer (b,d)
12. be able to detail molecular mechanisms
of the regulation of metabolism by hormones (a,b)
13. Understand fundamental techniques of
biotechnology and how they are applied to modern biomedicine, gene therapy and
genetic engineering (a,b,d)
14. Understand concepts underlying the
biochemical basis of neurobiology and applications to psychopharmacology (a,b)
15. Be able to think integratively with
respect to the fields of intermediary metabolism and molecular genetics (a,b)
16. Become familiar with modern trends in
biochemistry (b)
