Mark L. Steinberg
Professor
Biochemistry
Tel. (212) 650-8560
Fax: (212) 650-6092
E-mail: marste[at]sci.ccny.cuny.edu
B.A. University of Michigan
Ph.D. University of Pennsylvania
Research
The goal of our research is to understand the process by which human
epithelial cells come to acquire the properties of tumor cells derived
from malignant neoplasms. For this purpose this laboratory has utilized
a model system based upon infection of cultures of normal human epidermal
cells by the oncogenic virus, SV40. Three major aspects of transformation
in this system are currently under investigation: 1) alteration in the
structure and expression of endogenous oncogene sequences caused by
SV40 infection, 2) alteration in the structure and expression of the
SV40 virus transforming genes occurring after long term infection, and
3) SV40-induced expression of normally silent intermediate filament
genes as a model of altered gene expression in transformed epithelial
cells.
Publications
Morris, A., Steinberg, M.L. and Defendi, V.: Keratin gene expression
in SV40 transformed human keratinocytes. Proc. Natl. Acad. Sci., 82:8498-8502,
1985.
Chen,W., Ruml, T. and Steinberg, M.L.: Characterization of a 1.8
kb DNA segment located upstream from the human Ha-ras protooncogene
and possibly regulating its function. Gene, 155(2):253-256, 1995.
Guangtian Chen, G.T, Baez, J. and Steinberg, M.: integration of
the SV40 promoter/enhancer sequences in an anchorage independent clonal
subline of SV40-infected human keratinocytes. Gene, 183:41-45, 1996.
Steinberg, M. Immortalization of Human Epidermal Keratinocytes by
SV40. in Culture of Immortalized Cells. Chapter 5, pp. 95-120, Ian
Freshney ed., Wiley-Liss, New York, 1996
Vazquez, R. and Steinberg, M.L.: Enhancement of Genomic DNA Templates
for Polymerase Chain Reactions by Partial Restriction Digestion. Biotechniques
26:91-95, 1999.
Textbooks
Steinberg, M., Guyden, J., Calhoun, D., Coico, R. and Staiaino-Coico,
L. "Recombinant DNA Technology: Concepts and Biomedical Applications",
Prentice Hall, Englewood Cliffs, New Jersey Inc., 1993
"The Facts on File Dictionary of Biotechnology and Genetic Engineering",
Facts on File, Inc., New York, NY, contracted publication date: April,
1993